PlantTFDB
Plant Transcription Factor Database
v4.0
Previous version: v3.0
Transcription Factor Information
Basic Information | Signature Domain | Sequence | 
Basic Information? help Back to Top
TF ID AT2G18300.2
Common NameBHLH64, EN79, HBI1, T30D6.19
Organism
Taxonomic ID
Taxonomic Lineage
cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliophyta; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Family bHLH
Protein Properties Length: 337aa    MW: 37746.5 Da    PI: 6.1863
Description bHLH family protein
Gene Model
Gene Model ID Type Source Coding Sequence
AT2G18300.2genomeTAIRView CDS
Signature Domain? help Back to Top
Signature Domain
No. Domain Score E-value Start End HMM Start HMM End
1HLH25.42.5e-08196242555
                  HHHHHHHHHHHHHHHHHHHHCTSCCC...TTS-STCHHHHHHHHHHHHHHH CS
          HLH   5 hnerErrRRdriNsafeeLrellPkaskapskKlsKaeiLekAveYIksLq 55 
                  h+ +Er RR++i +++  L++++P +    +k   Ka +L + ++Y++ Lq
  AT2G18300.2 196 HSLAERARREKISKKMKYLQDIVPGC----NKVTGKAGMLDEIINYVQCLQ 242
                  999**********************9....677***************998 PP

Protein Features ? help Back to Top
3D Structure
Database Entry ID E-value Start End InterPro ID Description
SuperFamilySSF474592.49E-16189259IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
CDDcd000831.24E-6189246No hitNo description
PROSITE profilePS5088814.284191241IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene3DG3DSA:4.10.280.102.2E-16192260IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
PfamPF000105.1E-6196242IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
SMARTSM003533.4E-8197247IPR011598Myc-type, basic helix-loop-helix (bHLH) domain
Gene Ontology ? help Back to Top
GO Term GO Category GO Description
GO:0006355Biological Processregulation of transcription, DNA-templated
GO:0009299Biological ProcessmRNA transcription
GO:0009735Biological Processresponse to cytokinin
GO:0009740Biological Processgibberellic acid mediated signaling pathway
GO:0009742Biological Processbrassinosteroid mediated signaling pathway
GO:0009826Biological Processunidimensional cell growth
GO:0040008Biological Processregulation of growth
GO:0005634Cellular Componentnucleus
GO:0003700Molecular Functiontranscription factor activity, sequence-specific DNA binding
GO:0043425Molecular FunctionbHLH transcription factor binding
GO:0044212Molecular Functiontranscription regulatory region DNA binding
GO:0046983Molecular Functionprotein dimerization activity
Plant Ontology ? help Back to Top
PO Term PO Category PO Description
PO:0000013anatomycauline leaf
PO:0000037anatomyshoot apex
PO:0000230anatomyinflorescence meristem
PO:0000293anatomyguard cell
PO:0008019anatomyleaf lamina base
PO:0009006anatomyshoot system
PO:0009025anatomyvascular leaf
PO:0009046anatomyflower
PO:0009047anatomystem
PO:0020030anatomycotyledon
PO:0020038anatomypetiole
PO:0020100anatomyhypocotyl
PO:0020137anatomyleaf apex
PO:0025022anatomycollective leaf structure
PO:0001054developmental stagevascular leaf senescent stage
PO:0007064developmental stageLP.12 twelve leaves visible stage
PO:0007095developmental stageLP.08 eight leaves visible stage
PO:0007098developmental stageLP.02 two leaves visible stage
PO:0007103developmental stageLP.10 ten leaves visible stage
PO:0007115developmental stageLP.04 four leaves visible stage
PO:0007123developmental stageLP.06 six leaves visible stage
PO:0007611developmental stagepetal differentiation and expansion stage
Sequence ? help Back to Top
Protein Sequence    Length: 337 aa     Download sequence    Send to blast
MLEGLVSQES LSLNSMDMSV LERLKWVQQQ QQQLQQVVSH SSNNSPELLQ ILQFHGSNND  60
ELLESSFSQF QMLGSGFGPN YNMGFGPPHE SISRTSSCHM EPVDTMEVLL KTGEETRAVA  120
LKNKRKPEVK TREEQKTEKK IKVEAETESS MKGKSNMGNT EASSDTSKET SKGASENQKL  180
DYIHVRARRG QATDRHSLAE RARREKISKK MKYLQDIVPG CNKVTGKAGM LDEIINYVQC  240
LQRQVEFLSM KLAVLNPELE LAVEDVSVKQ FQAYFTNVVA SKQSIMVDVP LFPLDQQGSL  300
DLSAINPNQT TSIEAPSGSW ETQSQSLYNT SSLGFHY
Expression -- UniGene ? help Back to Top
UniGene ID E-value Expressed in
At.217710.0leaf
Expression -- Microarray ? help Back to Top
Source ID E-value
Genevisible265342_at0.0
Expression AtlasAT2G18300-
AtGenExpressAT2G18300-
ATTED-IIAT2G18300-
Expression -- Description ? help Back to Top
Source Description
UniprotTISSUE SPECIFICITY: Highly expressed in hypocotyls and cotyledons. Expressed in leaves, stems, and flowers. {ECO:0000269|PubMed:12679534, ECO:0000269|PubMed:23221598}.
Functional Description ? help Back to Top
Source Description
UniProtAtypical bHLH transcription factor that acts as positive regulator of cell elongation downstream of multiple external and endogenous signals by direct binding to the promoters and activation of the two expansin genes EXPA1 and EXPA8, encoding cell wall loosening enzymes. Transcriptional activity is inhibited when binding to the bHLH transcription factor IBH1. {ECO:0000269|PubMed:23221598}.
Function -- GeneRIF ? help Back to Top
  1. HBI1 is a positive regulator of BR-triggered responses, and the negative effect of PTI is likely due to the antagonism between BR and PTI signaling.
    [PMID: 24443525]
  2. HBI1, as a component of the central growth regulation circuit, functions as a major node of crosstalk that mediates a trade-off between growth and immunity in plants.
    [PMID: 24550223]
Binding Motif ? help Back to Top
Motif ID Method Source Motif file
MP00106PBM26531826Download
Motif logo
Cis-element ? help Back to Top
SourceLink
PlantRegMapAT2G18300.2
Regulation -- PlantRegMap ? help Back to Top
Source Upstream Regulator Target Gene
PlantRegMapRetrieveRetrieve
Regulation -- Hormone ? help Back to Top
Source Hormone
AHDcytokinin
Interaction ? help Back to Top
Source Intact With
BioGRIDAT1G30330
IntActSearch Q9ZPW3
Phenotype -- Mutation ? help Back to Top
Source ID
T-DNA ExpressAT2G18300
Annotation -- Nucleotide ? help Back to Top
Source Hit ID E-value Description
GenBankAF4120990.0AF412099.1 Arabidopsis thaliana At2g18300/T30D6.19 mRNA, complete cds.
GenBankAY0780300.0AY078030.1 Arabidopsis thaliana At2g18300/T30D6.19 mRNA, complete cds.
Annotation -- Protein ? help Back to Top
Source Hit ID E-value Description
RefseqNP_849976.10.0transcription factor bHLH64
SwissprotQ9ZPW30.0HBI1_ARATH; Transcription factor HBI1
TrEMBLF4IQH80.0F4IQH8_ARATH; Transcription factor bHLH64
STRINGAT2G18300.30.0(Arabidopsis thaliana)
Publications ? help Back to Top
  1. Riechmann JL, et al.
    Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.
    Science, 2000. 290(5499): p. 2105-10
    [PMID:11118137]
  2. Friedrichsen DM, et al.
    Three redundant brassinosteroid early response genes encode putative bHLH transcription factors required for normal growth.
    Genetics, 2002. 162(3): p. 1445-56
    [PMID:12454087]
  3. Heim MA, et al.
    The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.
    Mol. Biol. Evol., 2003. 20(5): p. 735-47
    [PMID:12679534]
  4. Toledo-Ortiz G,Huq E,Quail PH
    The Arabidopsis basic/helix-loop-helix transcription factor family.
    Plant Cell, 2003. 15(8): p. 1749-70
    [PMID:12897250]
  5. Yamada K, et al.
    Empirical analysis of transcriptional activity in the Arabidopsis genome.
    Science, 2003. 302(5646): p. 842-6
    [PMID:14593172]
  6. Bailey PC, et al.
    Update on the basic helix-loop-helix transcription factor gene family in Arabidopsis thaliana.
    Plant Cell, 2003. 15(11): p. 2497-502
    [PMID:14600211]
  7. Ohashi-Ito K,Kubo M,Demura T,Fukuda H
    Class III homeodomain leucine-zipper proteins regulate xylem cell differentiation.
    Plant Cell Physiol., 2005. 46(10): p. 1646-56
    [PMID:16081527]
  8. Hannah MA,Heyer AG,Hincha DK
    A global survey of gene regulation during cold acclimation in Arabidopsis thaliana.
    PLoS Genet., 2005. 1(2): p. e26
    [PMID:16121258]
  9. Brenner WG,Romanov GA,K
    Immediate-early and delayed cytokinin response genes of Arabidopsis thaliana identified by genome-wide expression profiling reveal novel cytokinin-sensitive processes and suggest cytokinin action through transcriptional cascades.
    Plant J., 2005. 44(2): p. 314-33
    [PMID:16212609]
  10. Truman W,de Zabala MT,Grant M
    Type III effectors orchestrate a complex interplay between transcriptional networks to modify basal defence responses during pathogenesis and resistance.
    Plant J., 2006. 46(1): p. 14-33
    [PMID:16553893]
  11. Osuna D, et al.
    Temporal responses of transcripts, enzyme activities and metabolites after adding sucrose to carbon-deprived Arabidopsis seedlings.
    Plant J., 2007. 49(3): p. 463-91
    [PMID:17217462]
  12. Kleine T,Kindgren P,Benedict C,Hendrickson L,Strand A
    Genome-wide gene expression analysis reveals a critical role for CRYPTOCHROME1 in the response of Arabidopsis to high irradiance.
    Plant Physiol., 2007. 144(3): p. 1391-406
    [PMID:17478635]
  13. Bi YM,Wang RL,Zhu T,Rothstein SJ
    Global transcription profiling reveals differential responses to chronic nitrogen stress and putative nitrogen regulatory components in Arabidopsis.
    BMC Genomics, 2007. 8: p. 281
    [PMID:17705847]
  14. Usadel B, et al.
    Global transcript levels respond to small changes of the carbon status during progressive exhaustion of carbohydrates in Arabidopsis rosettes.
    Plant Physiol., 2008. 146(4): p. 1834-61
    [PMID:18305208]
  15. Ascencio-Ib
    Global analysis of Arabidopsis gene expression uncovers a complex array of changes impacting pathogen response and cell cycle during geminivirus infection.
    Plant Physiol., 2008. 148(1): p. 436-54
    [PMID:18650403]
  16. Krishnaswamy SS, et al.
    Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana.
    BMC Plant Biol., 2008. 8: p. 91
    [PMID:18783601]
  17. Brady SM, et al.
    A stele-enriched gene regulatory network in the Arabidopsis root.
    Mol. Syst. Biol., 2011. 7: p. 459
    [PMID:21245844]
  18. Arabidopsis Interactome Mapping Consortium
    Evidence for network evolution in an Arabidopsis interactome map.
    Science, 2011. 333(6042): p. 601-7
    [PMID:21798944]
  19. Bai MY,Fan M,Oh E,Wang ZY
    A triple helix-loop-helix/basic helix-loop-helix cascade controls cell elongation downstream of multiple hormonal and environmental signaling pathways in Arabidopsis.
    Plant Cell, 2012. 24(12): p. 4917-29
    [PMID:23221598]
  20. Malinovsky FG, et al.
    Antagonistic regulation of growth and immunity by the Arabidopsis basic helix-loop-helix transcription factor homolog of brassinosteroid enhanced expression2 interacting with increased leaf inclination1 binding bHLH1.
    Plant Physiol., 2014. 164(3): p. 1443-55
    [PMID:24443525]
  21. Fan M, et al.
    The bHLH transcription factor HBI1 mediates the trade-off between growth and pathogen-associated molecular pattern-triggered immunity in Arabidopsis.
    Plant Cell, 2014. 26(2): p. 828-41
    [PMID:24550223]